graphing software graphpad prism 9.0  (GraphPad Software Inc)

Journal: Scientific Reports

Article Title: Stress exposure affects amyotrophic lateral sclerosis pathogenesis via PI3K/Akt and focal adhesion pathways: evidence from three experimental models

doi: 10.1038/s41598-025-02167-9

Figure Lengend Snippet: Chronic unpredicted mild stress effects in female and male ALS-predisposed mice. A-B. Rotarod and weight results in female h SOD1 G93A are showed at starting and end point in STRESSED and CTRL (not stressed) groups. Results are represented as median and quartile range of three mice per group, considering the average of three trials. Mixed-effects analysis followed by Uncorrected Fisher’s LDS analysis was used for statistical results. Rotarod of female h SOD1 G93A STRESSED at end point vs. female h SOD1 G93A STRESSED at starting point ** p < 0.01; weight of female h SOD1 G93A CTRL at end point vs. female h SOD1 G93A CTRL at starting point * p < 0.05. C-D. Rotarod and weight results in male h SOD1 G93A are described at starting and end point in STRESSED and CTRL (not stressed) groups. Results are represented as median and quartile range of three mice per groups, considering the average of three trials. Mixed-effects analysis followed by Uncorrected Fisher’s LDS analysis was used for statistical results. Weight of male h SOD1 G93A CTRL at end point vs. male h SOD1 G93A CTRL at starting point ** p < 0.01. E. Heatmap graph (created using GraphPad Prism version 10.1.2 for Windows) shows the relative gene expression in motor cortex and lumbar spinal cord samples of STRESSED groups normalized to CTRL (distinguishing female and male groups). Results are showed as red-to-green scale, using data of three mice per groups and indicating 1 (black) as baseline reference point of female or male h SOD1 G93A CTRL (not shown). Two-way ANOVA followed by Sidák’s multiple comparison test was used as statistical analysis and the significantly results were listed in Table .

Article Snippet: Two-way ANOVA followed by Tukey’s multiple comparisons test was used as statistical analysis and the details were listed in Table . (B) Heatmap graph (created using GraphPad Prism version 10.1.2 for Windows) shows the relative expression of genes of interest, in both CTRL and STRESSED experimental conditions.

Techniques: Gene Expression, Comparison

Journal: Scientific Reports

Article Title: Stress exposure affects amyotrophic lateral sclerosis pathogenesis via PI3K/Akt and focal adhesion pathways: evidence from three experimental models

doi: 10.1038/s41598-025-02167-9

Figure Lengend Snippet: Gene expression and relative PPI in CTRL and STRESSED cells. (A) Heatmap graph (created using GraphPad Prism version 10.1.2 for Windows) shows relative gene expression of NSC-34 h SOD1 WT STRESSED, NSC-34 h SOD1 G93A CTRL and STRESSED, normalized to NSC-34 h SOD1 WT CTRL. Results are showed as red-to-green scale of nine independent experiments, indicating 1 (black) as baseline of NSC-34 h SOD1 WT CTRL (not shown). Two-way ANOVA followed by Tukey’s multiple comparisons test was used as statistical analysis and the details were listed in Table . (B) Heatmap graph (created using GraphPad Prism version 10.1.2 for Windows) shows the relative expression of genes of interest, in both CTRL and STRESSED experimental conditions. Results are showed as red-to-green scale of nine independent experiments, indicating 1 (black) as baseline of NSC-34 h SOD1 WT (not shown). Unpaired t-test statistical analysis for each gene was used: NSC-34 h SOD1 G93A CTRL vs. NSC-34 h SOD1 WT CTRL, NSC-34 h SOD1 G93A STRESSED vs. NSC-34 h SOD1 WT STRESSED. C-D. Bar plot graphs show the genes significantly up-regulated and/or down-regulated, based on the unpaired t-test statistical analysis previously described in CTRL and STRESSED conditions. E-F. PPI, performed by STRING analysis, shows interactions among predicted proteins in CTRL and STRESSED conditions.

Article Snippet: Two-way ANOVA followed by Tukey’s multiple comparisons test was used as statistical analysis and the details were listed in Table . (B) Heatmap graph (created using GraphPad Prism version 10.1.2 for Windows) shows the relative expression of genes of interest, in both CTRL and STRESSED experimental conditions.

Techniques: Gene Expression, Expressing

Journal: Scientific Reports

Article Title: Stress exposure affects amyotrophic lateral sclerosis pathogenesis via PI3K/Akt and focal adhesion pathways: evidence from three experimental models

doi: 10.1038/s41598-025-02167-9

Figure Lengend Snippet: Chronic unpredicted mild stress effects in female and male ALS-predisposed mice. A-B. Rotarod and weight results in female h SOD1 G93A are showed at starting and end point in STRESSED and CTRL (not stressed) groups. Results are represented as median and quartile range of three mice per group, considering the average of three trials. Mixed-effects analysis followed by Uncorrected Fisher’s LDS analysis was used for statistical results. Rotarod of female h SOD1 G93A STRESSED at end point vs. female h SOD1 G93A STRESSED at starting point ** p < 0.01; weight of female h SOD1 G93A CTRL at end point vs. female h SOD1 G93A CTRL at starting point * p < 0.05. C-D. Rotarod and weight results in male h SOD1 G93A are described at starting and end point in STRESSED and CTRL (not stressed) groups. Results are represented as median and quartile range of three mice per groups, considering the average of three trials. Mixed-effects analysis followed by Uncorrected Fisher’s LDS analysis was used for statistical results. Weight of male h SOD1 G93A CTRL at end point vs. male h SOD1 G93A CTRL at starting point ** p < 0.01. E. Heatmap graph (created using GraphPad Prism version 10.1.2 for Windows) shows the relative gene expression in motor cortex and lumbar spinal cord samples of STRESSED groups normalized to CTRL (distinguishing female and male groups). Results are showed as red-to-green scale, using data of three mice per groups and indicating 1 (black) as baseline reference point of female or male h SOD1 G93A CTRL (not shown). Two-way ANOVA followed by Sidák’s multiple comparison test was used as statistical analysis and the significantly results were listed in Table .

Article Snippet: E. Heatmap graph (created using GraphPad Prism version 10.1.2 for Windows) shows the relative gene expression in motor cortex and lumbar spinal cord samples of STRESSED groups normalized to CTRL (distinguishing female and male groups).

Techniques: Gene Expression, Comparison

Journal: Scientific Reports

Article Title: Stress exposure affects amyotrophic lateral sclerosis pathogenesis via PI3K/Akt and focal adhesion pathways: evidence from three experimental models

doi: 10.1038/s41598-025-02167-9

Figure Lengend Snippet: Gene expression and relative PPI in CTRL and STRESSED cells. (A) Heatmap graph (created using GraphPad Prism version 10.1.2 for Windows) shows relative gene expression of NSC-34 h SOD1 WT STRESSED, NSC-34 h SOD1 G93A CTRL and STRESSED, normalized to NSC-34 h SOD1 WT CTRL. Results are showed as red-to-green scale of nine independent experiments, indicating 1 (black) as baseline of NSC-34 h SOD1 WT CTRL (not shown). Two-way ANOVA followed by Tukey’s multiple comparisons test was used as statistical analysis and the details were listed in Table . (B) Heatmap graph (created using GraphPad Prism version 10.1.2 for Windows) shows the relative expression of genes of interest, in both CTRL and STRESSED experimental conditions. Results are showed as red-to-green scale of nine independent experiments, indicating 1 (black) as baseline of NSC-34 h SOD1 WT (not shown). Unpaired t-test statistical analysis for each gene was used: NSC-34 h SOD1 G93A CTRL vs. NSC-34 h SOD1 WT CTRL, NSC-34 h SOD1 G93A STRESSED vs. NSC-34 h SOD1 WT STRESSED. C-D. Bar plot graphs show the genes significantly up-regulated and/or down-regulated, based on the unpaired t-test statistical analysis previously described in CTRL and STRESSED conditions. E-F. PPI, performed by STRING analysis, shows interactions among predicted proteins in CTRL and STRESSED conditions.

Article Snippet: E. Heatmap graph (created using GraphPad Prism version 10.1.2 for Windows) shows the relative gene expression in motor cortex and lumbar spinal cord samples of STRESSED groups normalized to CTRL (distinguishing female and male groups).

Techniques: Gene Expression, Expressing